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Procedure
for Testing the Pesticide’s Effectiveness Against Target Pests
To test the sample of the pesticide provided please
experiment as follow and you will be able to accurately determine:
A) Resulting biological activity against target pest of
different dilutions
B) Dilution required to obtain the desired level of
pest control
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Testing Procedures:
Collect pest and native vegetation and place in a
controlled environment to get an accurate count before proceeding to on-field
testing.
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Collect
a specified number of pests and place in a cage
a) Count and document the pest in the live stage of highest economic damage
b) Count and document the larvae of the pest if possible
c) Place native species of plants to be protected in each cage.
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Separate
the collected test sample of targeted pest and plants into 2 groups:
a)
Control group: place 20% of pests and larvae into cage.
Use no
pesticide in this cage
b) Test group: put 20% of pest samples including larvae into four separate
cages.
- Prepare 4 different
dilutions of the pesticide, some greater, some lesser than the suggested
dilutions of the “label” provided.
- Label clearly each cage as
“Control”, “1:100 dilution”, “1:200 dilution”, etc.
- Spray
with field equipment each test group cage with its corresponding dilution.
- Wait
for a few hours to 3 days, taking note of the mortality rate every so often,
then check each of the 5 cages.
- Count
the number of live and dead specimens and document.
- Make
a table of the results, including the Control group.
- Determine
the dilution desired based on the percentage of effectiveness.
- Prepare
the desired dilution and begin on-field testing.
- Study
field conditions, adjust dilutions, and spray crops as needed.
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Following the 3rd day of spraying and test
period of the pesticide, the number of target pests and/or larvae that did not
die may die later and/or may have been rendered incapable to reproduce. Count
both the control and test group cages several days after the analysis of the
first 3-day test. Normally, a field test will result in a higher mortality rate
due to the added stress on the pest from outdoor factors such as temperature and
weather changes (i.e. the heat of the day and the chill of the night). Also note
the activity levels of the Control group compared to the Test groups. The pests
that have been sprayed with the pesticide may appear more sluggish and less
responsive to stimuli, causing greater susceptibility to predators and other
outside factors.
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